Construction and Functional Evaluation of CRISPR/Cas9 Multiple Knockout Vectors of the FAD2 Gene Family

Soybean oil is a traditional edible oil. Increasing the oleic acid content an important direction of soybean breeding. The FAD2 family consists seven genes that regulate how converted into linoleic acid. Five gene family, GmFAD2-1B, GmFAD2-1A, GmFAD2-2B, GmFAD2-2C, and GmFAD2-2D, were taken as target in this study. Firstly, multivalent CRISPR/Cas9 gene-editing vector was constructed to expression. Multiple knockout vectors inserted varieties JN38, T6098, T7010 using Agrobacterium-mediated cotyledon transformation. functional analysis, agronomic character comparison mutant lines offspring different genotypes indicated JN38 significantly taller terms plant height than receptor JN38. fatty three groups showed same trend. contents plants higher those recipient plants, lower plants. best-performing among mutant, whose increased from 18.58% 54.07% decreased 57.79% 26.17%. In conclusion, expression multiple receptors.